Human line

FTlyo Respiratory pathogens 33

Overview

Ready to use lyophilised master mix (8-well strips each) for detection  of influenza A virus, influenza B virus, influenza C virus, influenza A(H1N1) virus (swine lineage), human parainfluenza viruses 1, 2, 3 and 4 ; human coronaviruses NL63, 229E, OC43 and HKU1; human metapneumoviruses A/B, human rhinovirus, human respiratory syncytial viruses A/B, human adenovirus, enterovirus, human parechovirus, human bocavirus, Pneumocystis jirovecii, Mycoplasma pneumoniae, Chlamydophila pneumoniaeStreptococcus pneumoniae, Haemophilus influenzae type B, Staphylococcus aureus,Moraxella catarrhalis, Bordetella spp.*, Klebsiella pneumoniae, Legionella pneumophila/ longbeachae, Salmonella spp., Haemophilus influenzae and internal control

*does not include Bordetella parapertussis

Principle

Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology

Targets

First lyo master mix:

  • influenza A virus
  • influenza B virus
  • influenza A(H1N1) virus (swine lineage)
  • human rhinovirus

Second lyo master mix:

  • human coronavirus NL63
  • human coronavirus 229E
  • human coronavirus OC43
  • human coronavirus HKU1

Third lyo master mix:

  • human parainfluenza 2
  • human parainfluenza 3
  • human parainfluenza 4
  • internal control

Fourth lyo master mix:

  • human parainfluenza 1
  • human metapneumoviruses A/B
  • human bocavirus
  • Mycoplasma pneumoniae

Fifth lyo master mix:

  • human respiratory syncytial viruses A/B
  • human adenovirus
  • enterovirus
  • human parechovirus

Sixth lyo master mix:

  • Chlamydophila pneumoniae
  • Streptococcus pneumoniae
  • Haemophilus influenzae B
  • Staphylococcus aureus

Seventh lyo master mix:

  • Klebsiella pneumoniae
  • Legionella pneumophila/ longbeachae
  • Salmonella spp.
  • Pneumocystis jirovecii

Eighth lyo master mix:

  • Moraxella catarrhalis
  • Bordetella spp. (except Bordetella parapertussis)
  • Haemophilus influenzae
  • influenza C virus

Specimen

This test is for use with extracted nucleic acid from respiratory samples (throat/nasal/nasopharyngeal swabs, bronchoalveolar lavage and sputum) of human origin

Storage

Liquid and lyophilised components: 2-8°C until expiration date

Sealing foils: room temperature

Shelf life

6 months minimum

Compatibility

To find out if the extraction method or qPCR thermocycler available in your facility is compatible with FTD assays, please see our full and detailed compatibility list under the “Resources” area or contact support@fast-trackdiagnostics.com