Gastroenteritis

2016 Article - "Molecular characterization of two rare human G8P[14] rotavirus strains, detected in Italy in 2012" (limited access)

Summary: We report the genomic characterization of two rare human G8P[14] rotavirus strains, identified in two children hospitalized with acute gastroenteritis in the southern Italian region of Apulia during rotavirus strain surveillance in 2012. These results reinforce the potential role of interspecies transmission and reassortment in generating novel rotavirus strains that might not be fully contrasted by current vaccines.

Nota Bene: Arrow Diagnostics, Genoa, Italy was part of the Fast-track diagnostics distributors' network.

Kit used: FTD Viral gastroenteritis
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2015 Poster - "Enteric virus screening: Multiplex RT-PCR and rapid immunochromatographic test evaluations using FTD Gastroenteritis assay" (pdf, 1MB)

Summary: This study Screened for NV GI and GII, SV, AstV, AdV and RV in 400 samples from adults and children at Torbay Hospital. using a Fast Track Diagnostics Gastroenteritis kit. The results found a high percentage of unrecognised viruses in samples entering the laboratory using previous testing protocols. Missing these leads to inappropriate patient treatments and is detrimental to infection control. ICT tests vary greatly in accuracy, but are rapid and relatively inexpensive. Multiplex PCR remains the definitive solution for providing hospitals with a highly sensitive and efficient method for patient diagnosis, management and infection control.

Kit used: FTD Viral gastroenteritis

2014 Poster - "Evaluation of Mswab direct nucleic acid extraction for detection of viruses, bacteria and parasites in faecal samples using Fast Track Diagnostics real-time multiplex kits" (pdf, 1MB)

Summary: This study evaluated Mswab direct nucleic acid extraction for detection of viruses, bacteria and parasites in faecal samples using Fast-track diagnostics real-time multiplex kits. It was found that The application of the MSwab direct nucleic acid extraction is time – and cost saving compared to commercial extraction methods and could be an alternative to our existing extraction method the NucliSENS® easyMAG. Especially for the total nucleic acid recovery of bacteria and parasites in combination with Fast-track diagnostics kits the method could be an alternative to existing extraction methodes. Optimization of the faecal specimen preparation will be investigated in the future.

Kits used: FTD Viral gastroenteritis, FTD EPA, FTD Stool parasites and FTD Bacterial gastroenteritis

2013 Article - "Systematic application of multiplex PCR enhances the detection of bacteria, parasites, and viruses in stool samples" (pdf, 233KB)

Summary: To determine whether systematic testing of faecal samples with a broad range multiplex PCR increases the diagnostic yield in patients with diarrhoea compared with conventional methods and a clinician initiated testing strategy. Using 1758 faecal samples from 1516 patients with diarrhoea submitted to two diagnostic laboratories were tested for viral, bacterial, and parasitic pathogens by Fast-Track Diagnostics multiplex real-time PCR kits and conventional diagnostic tests. It was found that using a systematic approach to the diagnosis of gastroenteritis improved diagnostic yield. This enhanced detection with PCR was achieved by a combination of improved detection of individual pathogens and detection of pathogens not requested or unable to be tested by conventional tests. This approach also allowed earlier identification for most pathogens and created a workflow which is likely to adapt well for many diagnostic laboratories.

Kit used: FTD Viral Gastroenteritis

2012 Poster - "Multiplex PCR Enhances the Detection of Viruses, Bacteria and Parasites in Gastroenteritis" (pdf, 606KB)

Summary: The aim of the study was to assess whether systematic testing of faecal samples with a broad range multiplex PCR increases the diagnostic yield in patients with diarrhoea. It was found that systematic application of a broad range PCR enhances the detection of gastroenteric pathogens. Workflow and turn-around time are favourable with this approach and cost barriers are reducing.

Kit used: FTD Viral gastroenteritis, FTD Bacterial gastroenteritis, FTD Stool parasites

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