2016 Poster - "Comparison of two exctraction methods: NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel) and NucliSENS® easyMag® (bioMérieux)" (pdf, 648KB)
Summary:The aim of this study was to compare two extraction methods, one semi-automated platform NucliSENS® easyMag® (bioMérieux) and one handprep extraction NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel). To confirm the compatibility of the performance of NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel) with FTD products we investigated nucleic acid extraction from 40 clinical specimens (human plasma, whole blood, swabs, faeces, culture and plasmids) and dilution series of certain samples. NucleoSpin® Blood was only tested with human plasma and whole blood, while NucleoSpin® DNA/RNA Virus Kit was validated with swab, faeces, culture, plasma and plasmids. For analysis of the extracted material commercial real-time multiplex assays from FTD (FTD Viral gastroenteritis, FTD Bacterial gastroenteritis, FTD Stool parasites, FTD ACE, FTD Respiratory pathogens 21 plus, FTD Viral meningitis, FTD Bacterial meningitis, FTD Hepatitis B DNA and FTD Urethritis basic) were performed on Applied BiosystemsTM 7500 (ThermoFisher Scientific) with extracted material from NucliSENS® easyMag® and with
extracted material from NucleoSpin® Blood and DNA/RNA Virus Kit.
Both were tested with Fast-track mastermix (FTD)