Generic publications

2016 Poster - "Comparison of two exctraction methods: NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel) and NucliSENS® easyMag® (bioMérieux)" (pdf, 648KB)

Summary:The aim of this study was to compare two extraction methods, one semi-automated platform NucliSENS® easyMag® (bioMérieux) and one handprep extraction NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel). To confirm the compatibility of the performance of NucleoSpin® Blood and NucleoSpin® DNA/RNA Virus Kit (Macherey-Nagel) with FTD products we investigated nucleic acid extraction from 40 clinical specimens (human plasma, whole blood, swabs, faeces, culture and plasmids) and dilution series of certain samples. NucleoSpin® Blood was only tested with human plasma and whole blood, while NucleoSpin® DNA/RNA Virus Kit was validated with swab, faeces, culture, plasma and plasmids. For analysis of the extracted material commercial real-time multiplex assays from FTD (FTD Viral gastroenteritis, FTD Bacterial gastroenteritis, FTD Stool parasites, FTD ACE, FTD Respiratory pathogens 21 plus, FTD Viral meningitis, FTD Bacterial meningitis, FTD Hepatitis B DNA and FTD Urethritis basic) were performed on Applied BiosystemsTM 7500 (ThermoFisher Scientific) with extracted material from NucliSENS® easyMag® and with extracted material from NucleoSpin® Blood and DNA/RNA Virus Kit.

Both were tested with Fast-track mastermix (FTD)

2015 Poster - "Lyophilised real-time PCR kits from Fast-track diagnostics: faster and even easier" (pdf, 445KB)

Summary: The easy to use application of lyophilised Fast-track diagnostics real-time multiplexes is a time saving and more flexible alternative compared to our existing liquid Fast-track diagnostics real-time multiplexes. The simple addition of extracted material to the lyophilised reagent allows people to save time and improve flexibility by testing anything between 1 and 62 patients without discarding any reagent afterwards.

Kits used: FTD Bacterial gastroenteritis, FTD ACE and FTD Respiratory pathogens 21

2012 Poster - "Comparison of two extraction methods for detection of viruses, bacteria" (pdf, 873KB)

Summary: The study obtained just small differences (less or equal 1 log Ct range) in the recovery of nucleic acid for all of the tested pathogens. For Gram-pos bacteria (Spneu and Saur) they received the same range of linearity with both tested methods. Also for the Gram-neg bacteria (Kpneu and Morax) the linear range was equal for both extraction methods. Except for Cpneu where the last dilution step with the RTP® Pathogens kit was missed. In general the outcome of the RTP® Pathogens kit was equal or slightly more efficient than the nucleic acid isolation with the easyMAG. It is clear that, as long as one selects an extraction kit which is designed for the required pathogens, that there is little to choose between many kits on the market (two examples are compared here). However, for an integrated infection laboratory, it is crucial that extraction kits are chosen that are equally efficient for all types of pathogens. The major decision now facing laboratories is not extraction, but sensitivity of the downstream nucleic acid detection system.

Kits used: FTD Viral gastroenteritis, FTD EPA, FTD Stool parasites, FTD Bacterial gastroenteritis, FTD Respiratory pathogens 21 and FTD Respiratory pathogens 33

Loading...