Products

FTD Eye

Overview

Two tube multiplex for detection of herpes simplex virus 1, 2, varicella-zoster virus, adenovirus, Chlamydia trachomatis and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • Herpes simplex virus 1
  • Herpes simplex virus 2
  • Varicella-Zoster virus
  • Internal control

Second tube multiplex PCR:

  • Chlamydia trachomatis
  • Adenovirus (AV)
  • Internal control

Specimen

This test is for use with extracted nucleic acid from eye swabs of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix, a one-step qRT-PCR kit.


To find out if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix (Fast-track Diagnostics) and AgPath ID™ One-Step RT-PCR kit (life technologies™).


 To know if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, confer to info@fast-trackdiagnostics.com!