Products

FTD Respiratory pathogens 21 plus

Overview

Six tube multiplex for detection of influenza A, influenza A (H1N1) swl, influenza B, rhinovirus, coronavirus NL63, 229E, OC43, HKU1, parainfluenza 1, 2, 3, 4, human metapneumovirus A/B, bocavirus, respiratory syncytial virus A/B, adenovirus, enterovirus, parechovirus, Mycoplasma pneumoniaeChlamydia pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae and Haemophilus influenzae type B and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • Influenza A
  • Influenza B
  • Influenza A H1N1
  • Rhinovirus

Second tube multiplex PCR:

  • Coronavirus NL63
  • Coronavirus 229E
  • Coronavirus OC43
  • Coronavirus HKU1

Third tube multiplex PCR:

  • Parainfluenza 2
  • Parainfluenza 3
  • Parainfluenza 4
  • Internal Control

Fourth tube multiplex PCR:

  • Parainfluenza 1
  • Human metapneumovirus A/B
  • Bocavirus
  • Mycoplasma pneumoniae

Fifth tube multiplex PCR:

  • Respiratory Syncytial viruses A/B
  • Adenovirus
  • Enterovirus
  • Parechovirus

Sixth tube multiplex PCR:

  • Chlamydia pneumoniae
  • Streptococcus pneumoniae
  • Haemophilus influenzae type B
  • Staphylococcus aureus

Specimen

This test is for use with extracted RNA and DNA from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix, a one-step qRT-PCR kit.


To find out if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com