Products

FTD Respiratory pathogens 33

Overview

Eight tube multiplex for detection of repiratory pathogens: influenza A ; influenza B ; influenza C ; influenza A (H1N1) swl ; parainfluenza viruses 1, 2, 3 and 4 ; coronaviruses NL63, 229E, OC43 and HKU1 ; human metapneumoviruses A/B ; rhinovirus ; respiratory syncytial viruses A and B ; adenovirus ; enterovirus ; parechovirus; bocavirus ; Pneumocystis jirovecii ; Mycoplasma pneumoniae ; Chlamydia pneumoniae ; Streptococcus pneumoniae ; Haemophilus influenzae type B ; Staphylococcus aureus ; Moraxella catarrhalis ; Bordetella spp.* ; Klebsiella pneumoniae ; Legionella pneumophila/ Legionella longbeachae ; Salmonella species and Haemophilus influenzae including internal control.

*does not include Bordetella parapertussis

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • Influenza A (FluA)
  • Influenza B (FluB)
  • Influenza A  (H1N1) swl
  • Rhinovirus

Second tube multiplex PCR:

  • Coronavirus NL63
  • Coronavirus 229E
  • Coronavirus OC43
  • Coronavirus HKU1

Third tube multiplex PCR:

  • Parainfluenza 2
  • Parainfluenza 3
  • Parainfluenza 4
  • Internal Control

Fourth tube multiplex PCR:

  • Parainfluenza 1
  • Human metapneumovirus A/B
  • Bocavirus
  • Mycoplasma pneumoniae

Fifth tube multiplex PCR:

  • Respiratory syncytial viruses A/B
  • Adenovirus
  • Enterovirus
  • Parechovirus

Sixth tube multiplex PCR:

  • Chlamydia pneumoniae
  • Streptococcus pneumoniae
  • Haemophilus influenzae type B
  • Staphylococcus aureus

Seventh tube multiplex PCR:

  • Klebsiella pneumoniae
  • Legionella pneumophila/Legionella longbeachae
  • Salmonella spp
  • Pneumocystis jirovecii

Eighth tube multiplex PCR:

  • Moraxella catarrhalis
  • Bordetella spp. (except Bordetella parapertussis)
  • Haemophilus influenzae
  • Influenza C (FluC)

Specimen

This test is for use with extracted RNA and DNA from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix, a one-step qRT-PCR kit.


To find out if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com