Products

FTD SPn/Staph/MC/HI

Overview

One tube multiplex for detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

One tube multiplex PCR:

  • Streptococcus pneumoniae (Spn)
  • Haemophilus influenzae (Hi)
  • Moraxella catarrhalis (Hc)
  • Staphylococcus aureus (Staph)

Specimen

This test is for use with extracted nucleic acid from throat/nasal swabs, bronchoalveolar lavage, sputum and culture of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus/Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • Duplicα®Prep (EuroClone)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix, a one-step qRT-PCR kit.


To find out if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com