Products

FTD Tropical fever core

Overview

Two tube multiplex for detection of dengue virus, chikungunya virus, West Nile virus, Plasmodium spp., Rickettsia spp., Leptospira spp., Salmonella spp. and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • Dengue virus
  • Rickettsia spp.
  • Salmonella spp.
  • West Nile virus

Second tube multiplex PCR:

  • Plasmodium spp.
  • Chikungunya
  • Leptospira spp.
  • Internal control

Specimen

This test is for use with extracted nucleic acid from whole blood or urine samples of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix (Fast-track Diagnostics) and AgPath ID™ One-Step RT-PCR kit (life technologies™).


To know if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, confer to info@fast-trackdiagnostics.com!