Products

FTD Zika/Dengue/Chik

Overview

Two tube multiplex for detection of zika virus, dengue virus, chikungunya virus and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • Zika virus
  • Internal control

Second tube multiplex PCR:

  • Dengue virus
  • Chikungunya virus
  • Internal control

Specimen

This test is for use with extracted nucleic acid from serum/plasma or urine of human origin. For dengue virus and chikungunja virus, whole blood can also be used.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix (Fast-track Diagnostics) and AgPath ID™ One-Step RT-PCR kit (life technologies™).


To know if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, confer to info@fast-trackdiagnostics.com!