Products

FTlyo Bacterial meningitis

Overview

Ready to use lyo master mix (8-well strips) for detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae including an internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology

Targets

Lyo master mix:

  • Neisseria meningitidis
  • Streptococcus pneumoniae
  • Haemophilus influenza
  • Internal control

Specimen

This test is for use with extracted nucleic acid from cerebrospinal fluid (CSF) and EDTA or citrated blood samples of human origin

Storage

Liquid components: -20°C

Lyophilized components: 2-8°C

Shelf life

12 months

Equipment

 

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • RTP® Pathogen Kit (STRATEC Biomedical AG)
  • RTP® Bacteria DNA Mini Kit (STRATEC Biomedical AG)
  • Duplicα ® Prep (EuroClone®)
  • Maxwell® (Promega)
  • QIAamp DNA Stool Mini Kit (Qiagen)
  • QIAamp MiniElute Virus spin (Qiagen)
  • QIAamp Viral RNA Mini (Qiagen)
  • MagMax™ (Thermo Fisher Scientific)
  • QIAsymphony® SP instrument (Qiagen)
  • Arrow/ LIAISON®IXT (DiaSorin), SeePrep12™
  • Magtration® System 12GC PLUS (PSS®)

 

AMPLIFICATION:

Cycler

FTlyo 28

Applied Biosystems 7500 (Thermo Fisher Scientific) H/T 
 Applied Biosystems 7500 fast  (Thermo Fisher Scientific)
 Applied Biosystems ViiA™7  (Thermo Fisher Scientific) H/T 
 Rotor-Gene 6000 (Qiagen)
 Rotor-Gene 3000 (Qiagen)
 Rotor-Gene Q (Qiagen)
 LightCycler® 480 (Roche)
 CFX96™ (Bio-Rad) L/T
 DX (Bio-Rad™) L/T
SmartCycler® (Cepheid)

H= high profile strips ; L= low profile strips ; T= Transfer of reaction mix to according plastics 

 

To find out if the extraction method, qPCR cycler or enzyme available in your facility is compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com