Products

FTlyo MERS-CoV

Overview

Two ready to use lyo master mixes (8-well strips each) for detection and confirmation of Betacoronavirus hCoV-EMC 2012 (MERS-CoV) including an internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology

Targets

First lyo mastermix:

  • MERS-CoV screening (1st target gene)
  • internal control

Second lyo mastermix:

  • MERS-CoV confirmation (2nd target gene)

Specimen

This test is for use with extracted RNA from respiratory samples (throat/nasal swabs, Broncho alveolar lavage (BAL) and sputum) of human origin

Storage

Liquid components: -20°C

Lyophilized components: 2-8°C

Shelf life

12 months

Equipment

 

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • RTP® Pathogen Kit (STRATEC Biomedical AG)
  • RTP® Bacteria DNA Mini Kit (STRATEC Biomedical AG)
  • Duplicα ® Prep (EuroClone®)
  • Maxwell® (Promega)
  • QIAamp DNA Stool Mini Kit (Qiagen)
  • QIAamp MiniElute Virus spin (Qiagen)
  • QIAamp Viral RNA Mini (Qiagen)
  • MagMax™ (Thermo Fisher Scientific)
  • QIAsymphony® SP instrument (Qiagen)
  • Arrow/ LIAISON®IXT (DiaSorin), SeePrep12™
  • Magtration® System 12GC PLUS (PSS®)

 

AMPLIFICATION:

Cycler

FTlyo 50.1
Applied Biosystems 7500 (Thermo Fisher Scientific)  H/T
Applied Biosystems 7500 fast  (Thermo Fisher Scientific)
Applied Biosystems ViiA™7  (Thermo Fisher Scientific) H/T 
Rotor-Gene 6000 (Qiagen)
Rotor-Gene 3000 (Qiagen)
Rotor-Gene Q (Qiagen)
LightCycler® 480 (Roche) L/T 
CFX96™ (Bio-Rad) L/T
DX (Bio-Rad™) L/T
SmartCycler® (Cepheid)

H= high profile strips ; L= low profile strips ; T= Transfer of reaction mix to according plastics 

 

To find out if the extraction method, qPCR cycler or enzyme available in your facility is compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com