Human line

FTD Respiratory pathogens 33

Overview

Eight tube multiplex for detection of influenza A virus, influenza B virus, influenza C virus, influenza A(H1N1) swl virus, human parainfluenza viruses 1, 2, 3 and 4 ; human coronaviruses NL63, 229E, OC43 and HKU1; human metapneumoviruses A/B, human rhinovirus, human respiratory syncytial viruses A/B, human adenovirus, enterovirus, human parechovirus, human bocavirus, Pneumocystis jirovecii,Mycoplasma pneumoniae,Chlamydia pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae type B, Staphylococcus aureus,Moraxella catarrhalis, Bordetella spp.*, Klebsiella pneumoniae, Legionella pneumophila/ longbeachae, Salmonella spp., Haemophilus influenzae and internal control

*does not include Bordetella parapertussis

Principle

Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology

Targets

First tube multiplex PCR:

  • influenza A virus
  • influenza B virus
  • influenza A(H1N1) swl virus
  • human rhinovirus

Second tube multiplex PCR:

  • human coronavirus NL63
  • human coronavirus 229E
  • human coronavirus OC43
  • human coronavirus HKU1

Third tube multiplex PCR:

  • human parainfluenza 2
  • human parainfluenza 3
  • human parainfluenza 4
  • internal Control

Fourth tube multiplex PCR:

  • human parainfluenza 1
  • human metapneumoviruses A/B
  • human bocavirus
  • Mycoplasma pneumoniae

Fifth tube multiplex PCR:

  • human respiratory syncytial viruses A/B
  • human adenovirus
  • enterovirus
  • human parechovirus

Sixth tube multiplex PCR:

  • Chlamydia pneumoniae
  • Streptococcus pneumoniae
  • Haemophilus influenzae B
  • Staphylococcus aureus

Seventh tube multiplex PCR:

  • Klebsiella pneumoniae
  • Legionella pneumophila/ longbeachae
  • Salmonella spp.
  • Pneumocystis jirovecii

Eighth tube multiplex PCR:

  • Moraxella catarrhalis
  • Bordetella spp. (except Bordetella parapertussis)
  • Haemophilus influenzae
  • influenza C virus

Specimen

This test is for use with extracted RNA and DNA from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin

Storage

-20°C

Shelf life

12 months from manufacture

Compatibility

To find out if the extraction method or qPCR thermocycler available in your facility is compatible with FTD assays, please see our full and detailed compatibility list under the “Resources” area or contact support@fast-trackdiagnostics.com