Products

FTD BKV

Overview

One tube multiplex for detection AND quantification of BKV and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

One tube multiplex PCR:

  • BK virus
  • Internal control

Specimen

The quantitative validation of FTD BKV was done with extracted nucleic acid from human EDTA or Citrate plasma with NucliSENS® easyMag® and ABI® 7500.

In clinical performance evaluation studies the test was also successfully qualitatively used with human urine, EDTA blood, plasma and serum.

In case human urine, EDTA blood or serum are intended to use for quantitative analysis, the procedure has to be validated by the user.

Storage

-20° C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus/Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • Duplicα®Prep (EuroClone)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)


To find out if the extraction method or qPCR cycler available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com