Products

FTD Dermatophytes

Overview

Two tube multiplex for detection of Trichophyton mentagrophytes complex, Trichophyton tonsurans, Trichophyton violaceum, Trichophyton rubrum species complex, Microsporum canis, Microsporum audouinii, Microsporum ferrugineum and internal control.

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology.

Targets

First tube multiplex PCR:

  • T. mentagrophytes complex
  • T. tonsurans
  • T. violaceum
  • Internal control

 

Second tube multiplex PCR:

  • Trichophyton rubrum complex
  • M. canis
  • M. audouinii

Specimen

This test is for use with extracted nucleic acid from skin scale specimens, plucked hair, nails, culture or swabs of human origin.

Storage

-20°C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • QIAamp Mini Elute virus Spin or QIAamp viral RNA kit - manual or in combination with QIAcube (Qiagen)
  • QIAsymphony SP in combination with DSP Virus / Pathogen and the DNA Mini kit (Qiagen)
  • RTP® Pathogen kit (STRATEC)
  • DuplicαPrep (EuroClone®)
  • Maxwell® 16 Tissue LEV Total (stool samples) & Maxwell® 16 Buccal Swab LEV (Promega)

AMPLIFICATION:

  • Applied Biosystems® 7500 / 7500 Fast (life technologies™)
  • Rotor-Gene 3000 / 6000 / Q (Qiagen)
  • CFX96® / DX® with CFX software (BIO-RAD)
  • LightCycler® 480 (Roche)
  • SmartCycler® with Life Science software 2.0d (Cepheid)

ENZYME:
The test is fully validated with fast-track mastermix, a one-step qRT-PCR kit.


To find out if the extraction method, qPCR cycler or enzyme available in your facility are compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com