Products

FTD West Nile virus

Overview

One tube multiplex for detection of West Nile virus and internal control

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology

Targets

One tube multiplex PCR:

  • West Nile virus
  • internal control

Specimen

This test is for use with extracted nucleic acid from plasma or urine samples of human origin. This test is not suitable for blood donor screening

Storage

-20° C

Shelf life

12 months

Equipment

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • RTP® Pathogen Kit (STRATEC Biomedical AG)
  • RTP® Bacteria DNA Mini Kit (STRATEC Biomedical AG)
  • Duplicα ® Prep (EuroClone®)
  • Maxwell® (Promega)
  • QIAamp MiniElute Virus spin (Qiagen)
  • QIAamp Viral RNA Mini (Qiagen)
  • MagMax™ (Thermo Fisher Scientific)
  • QIAsymphony® SP instrument (Qiagen)
  • Arrow/ LIAISON®IXT (DiaSorin), SeePrep12™
  • Magtration® System 12GC PLUS (PSS®)

AMPLIFICATION:

  • Applied Biosystems 7500 (Thermo Fisher Scientific)
  • Applied Biosystems 7500 fast  (Thermo Fisher Scientific)
  • Applied Biosystems ViiA™7  (Thermo Fisher Scientific)
  • Rotor-Gene 6000 (Qiagen)
  • Rotor-Gene 3000 (Qiagen)
  • Rotor-Gene Q (Qiagen)
  • LightCycler® 480 (Roche)
  • CFX96™ (Bio-Rad)
  • DX (Bio-Rad™)
  • SmartCycler® (Cepheid)

ENZYME:
The test is fully validated with Fast-track mastermix, a one-step qRT-PCR kit


To find out if the extraction method, qPCR cycler or enzyme available in your facility is compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com