Products

FTlyo Bacterial gastroenteritis

Overview

Two ready to use lyo master mixes (8-well strips each) for detection of Campylobacter coli/jejuni/lari, Clostridium difficile, Escherichia coli verotoxin positives, Salmonella spp., Shigella spp., enteroinvasive Escherichia coli, Yersinia enterocolitica and internal control

Principle

Multiplex Real-Time PCR for detection of pathogen genes by TaqMan® technology

Targets

First lyo mastermix:

  • Campylobacter
  • verotoxin producing Escherichia coli
  • internal control

Second lyo mastermix:

  • Salmonella
  • Shigella/ enteroinvasive Escherichia coli
  • Yersinia enterocolitica
  • Clostridium difficile 

Specimen

This test is for use with extracted nucleic acid from stool samples of human origin

Storage

Liquid components: -20°C

Lyophilized components: 2-8°C

Shelf life

12 months

Equipment

 

EXTRACTION:

  • NucliSENS® easyMAG® (bioMérieux)
  • RTP® Pathogen Kit (STRATEC Biomedical AG)
  • RTP® Bacteria DNA Mini Kit (STRATEC Biomedical AG)
  • Duplicα ® Prep (EuroClone®)
  • Maxwell® (Promega)
  • QIAamp MiniElute Virus spin (Qiagen)
  • QIAamp MiniElute Virus spin (Qiagen)
  • QIAamp Viral RNA Mini (Qiagen)
  • MagMax™ (Thermo Fisher Scientific)
  • QIAsymphony® SP instrument (Qiagen)
  • Arrow/ LIAISON®IXT (DiaSorin), SeePrep12™
  • Magtration® System 12GC PLUS (PSS®)

 

AMPLIFICATION:

Cycler

FTlyo 14.1 

Applied Biosystems 7500 (Thermo Fisher Scientific)  H/T
 Applied Biosystems 7500 fast  (Thermo Fisher Scientific)
 Applied Biosystems ViiA™7  (Thermo Fisher Scientific) H/T 
 Rotor-Gene 6000 (Qiagen)
 Rotor-Gene 3000 (Qiagen)
 Rotor-Gene Q (Qiagen)
 LightCycler® 480 (Roche) L/T 
 CFX96™ (Bio-Rad) L/T
 DX (Bio-Rad™) L/T
SmartCycler® (Cepheid)

H= high profile strips ; L= low profile strips ; T= Transfer of reaction mix to according plastics 

 

To find out if the extraction method, qPCR cycler or enzyme available in your facility is compatible with our tests, please see our compatibility list or contact support@fast-trackdiagnostics.com